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The advent of the PAM FACS technique revolutionized the method for measuring DNA content. Now, using a strain of budding yeast, scientists have demonstrated a microfluidic method for measuring DNA content that can be directly applied to a variety of cell samples. Published in the Proceedings of the National Academy of Sciences, the new method offers several advantages over existing flow cytometry techniques for measuring DNA content: it requires only 10 picograms of DNA and it can be used for virtually any cell type, even difficult to grow cell lines. The method is rapid, straightforward and can be performed in a microfluidic device.
The successful development of a highly specific real-time PCR assay [Fluorescence Resonance Energy Transfer (FRET)] using the Mer-FRET/YFP-labeled reagents demonstrates a rapid, specific and sensitive format for quantifying HIV-1 gp41-specific FRET signal in a clinical specimen. The development of this assay represents a novel approach to HIV-1 quantification that may provide a standardized, fingertip test for monitoring the course of HIV infection and for assessing its treatment. This may lead to broader access to antiviral therapy in resource-poor and resource-limited settings. 1) The CFP, YFP, and Mer-FRET/YFP-labeled oligonucleotide primers of the HIV-1gp41 real-time PCR assay were validated. d2c66b5586
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